pngase f solution Search Results


pngase-f solution (pomega, germany, recombinant, cloned from elizabethkingia miricola 10 u/μl)  (Pomega Inc)
90
Pomega Inc pngase-f solution (pomega, germany, recombinant, cloned from elizabethkingia miricola 10 u/μl)
Pngase F Solution (Pomega, Germany, Recombinant, Cloned From Elizabethkingia Miricola 10 U/μl), supplied by Pomega Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pngase+f+solution/pngase+f+solution/pmc06851832__ANIE___58___11631___s001-244-11-13
Average 90 stars, based on 1 article reviews
pngase-f solution (pomega, germany, recombinant, cloned from elizabethkingia miricola 10 u/μl) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
0.1 μg/μl pngase f solution  (N-Zyme BioTec GmbH)
90
N-Zyme BioTec GmbH 0.1 μg/μl pngase f solution
(A) Non-arthritic Cadaveric Joint (control) cadaveric tissue donors without diagnosed arthritis, osteoporosis, or fracture, and Osteoarthritis (OA) patients receiving knee replacement compose a cohort that is age, sex, and BMI matched and stratified by their clinical OARSI (arthritis) grade. Safranin-O/Fast Green histologic staining (B) of the tibial plateau of a non-arthritic donor (Control - top) and a representative patient with Osteoarthritis (OA - bottom) depict the loss of proteoglycan staining (red) in Medial OA, indicative of cartilage loss. Separation of tissue into medial and lateral sections allows for both site-matched comparisons with cadaveric tissue (medial OA vs Medial Control, blue arrow) and tissue-matched comparisons to healthier tissue from the same OA patients (OA Medial vs OA Lateral, red arrow). Formalin-fixed, paraffin-embedded (FFPE) tissue sections are prepared (C) for MALDI-MSI spatial proteomics including deparaffinization and epitope retrieval prior to <t>PNGase</t> <t>F</t> enzyme treatment for removal of proteoglycans. Tissues are matrix-coated and imaged for glycomics before being washed and prepared for peptide imaging following a similar protocol utilizing collagenase III that targets the skeletal ECM. Following matrix application, slides are imaged (D) with the timsTOF fleX mass spectrometer (Bruker).
0.1 μg/μl Pngase F Solution, supplied by N-Zyme BioTec GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pngase+f+solution/0+1+%CE%BCg+%CE%BCl+pngase+f+solution/bio_rxiv__2024__08__03__606482-311-8-11
Average 90 stars, based on 1 article reviews
0.1 μg/μl pngase f solution - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
pngase f  (Catalent Pharma Solutions)
90
Catalent Pharma Solutions pngase f
(A) Non-arthritic Cadaveric Joint (control) cadaveric tissue donors without diagnosed arthritis, osteoporosis, or fracture, and Osteoarthritis (OA) patients receiving knee replacement compose a cohort that is age, sex, and BMI matched and stratified by their clinical OARSI (arthritis) grade. Safranin-O/Fast Green histologic staining (B) of the tibial plateau of a non-arthritic donor (Control - top) and a representative patient with Osteoarthritis (OA - bottom) depict the loss of proteoglycan staining (red) in Medial OA, indicative of cartilage loss. Separation of tissue into medial and lateral sections allows for both site-matched comparisons with cadaveric tissue (medial OA vs Medial Control, blue arrow) and tissue-matched comparisons to healthier tissue from the same OA patients (OA Medial vs OA Lateral, red arrow). Formalin-fixed, paraffin-embedded (FFPE) tissue sections are prepared (C) for MALDI-MSI spatial proteomics including deparaffinization and epitope retrieval prior to <t>PNGase</t> <t>F</t> enzyme treatment for removal of proteoglycans. Tissues are matrix-coated and imaged for glycomics before being washed and prepared for peptide imaging following a similar protocol utilizing collagenase III that targets the skeletal ECM. Following matrix application, slides are imaged (D) with the timsTOF fleX mass spectrometer (Bruker).
Pngase F, supplied by Catalent Pharma Solutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pngase+f+solution/pngase+f/pmc06601568-303-18-9
Average 90 stars, based on 1 article reviews
pngase f - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
n-glycosidase f (pngase f) solution  (SunChrom Inc)
90
SunChrom Inc n-glycosidase f (pngase f) solution
(A) Non-arthritic Cadaveric Joint (control) cadaveric tissue donors without diagnosed arthritis, osteoporosis, or fracture, and Osteoarthritis (OA) patients receiving knee replacement compose a cohort that is age, sex, and BMI matched and stratified by their clinical OARSI (arthritis) grade. Safranin-O/Fast Green histologic staining (B) of the tibial plateau of a non-arthritic donor (Control - top) and a representative patient with Osteoarthritis (OA - bottom) depict the loss of proteoglycan staining (red) in Medial OA, indicative of cartilage loss. Separation of tissue into medial and lateral sections allows for both site-matched comparisons with cadaveric tissue (medial OA vs Medial Control, blue arrow) and tissue-matched comparisons to healthier tissue from the same OA patients (OA Medial vs OA Lateral, red arrow). Formalin-fixed, paraffin-embedded (FFPE) tissue sections are prepared (C) for MALDI-MSI spatial proteomics including deparaffinization and epitope retrieval prior to <t>PNGase</t> <t>F</t> enzyme treatment for removal of proteoglycans. Tissues are matrix-coated and imaged for glycomics before being washed and prepared for peptide imaging following a similar protocol utilizing collagenase III that targets the skeletal ECM. Following matrix application, slides are imaged (D) with the timsTOF fleX mass spectrometer (Bruker).
N Glycosidase F (Pngase F) Solution, supplied by SunChrom Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pngase+f+solution/n+glycosidase+f++pngase+f++solution/pm28891400-98-0-45
Average 90 stars, based on 1 article reviews
n-glycosidase f (pngase f) solution - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
pngase f solution (20 μl in 200 μl water)  (GSI Creos Corporation)
90
GSI Creos Corporation pngase f solution (20 μl in 200 μl water)
(A) Non-arthritic Cadaveric Joint (control) cadaveric tissue donors without diagnosed arthritis, osteoporosis, or fracture, and Osteoarthritis (OA) patients receiving knee replacement compose a cohort that is age, sex, and BMI matched and stratified by their clinical OARSI (arthritis) grade. Safranin-O/Fast Green histologic staining (B) of the tibial plateau of a non-arthritic donor (Control - top) and a representative patient with Osteoarthritis (OA - bottom) depict the loss of proteoglycan staining (red) in Medial OA, indicative of cartilage loss. Separation of tissue into medial and lateral sections allows for both site-matched comparisons with cadaveric tissue (medial OA vs Medial Control, blue arrow) and tissue-matched comparisons to healthier tissue from the same OA patients (OA Medial vs OA Lateral, red arrow). Formalin-fixed, paraffin-embedded (FFPE) tissue sections are prepared (C) for MALDI-MSI spatial proteomics including deparaffinization and epitope retrieval prior to <t>PNGase</t> <t>F</t> enzyme treatment for removal of proteoglycans. Tissues are matrix-coated and imaged for glycomics before being washed and prepared for peptide imaging following a similar protocol utilizing collagenase III that targets the skeletal ECM. Following matrix application, slides are imaged (D) with the timsTOF fleX mass spectrometer (Bruker).
Pngase F Solution (20 μl In 200 μl Water), supplied by GSI Creos Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pngase+f+solution/pngase+f+solution++20+%CE%BCl+in+200+%CE%BCl+water+/pm33710478-66-0-19
Average 90 stars, based on 1 article reviews
pngase f solution (20 μl in 200 μl water) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


(A) Non-arthritic Cadaveric Joint (control) cadaveric tissue donors without diagnosed arthritis, osteoporosis, or fracture, and Osteoarthritis (OA) patients receiving knee replacement compose a cohort that is age, sex, and BMI matched and stratified by their clinical OARSI (arthritis) grade. Safranin-O/Fast Green histologic staining (B) of the tibial plateau of a non-arthritic donor (Control - top) and a representative patient with Osteoarthritis (OA - bottom) depict the loss of proteoglycan staining (red) in Medial OA, indicative of cartilage loss. Separation of tissue into medial and lateral sections allows for both site-matched comparisons with cadaveric tissue (medial OA vs Medial Control, blue arrow) and tissue-matched comparisons to healthier tissue from the same OA patients (OA Medial vs OA Lateral, red arrow). Formalin-fixed, paraffin-embedded (FFPE) tissue sections are prepared (C) for MALDI-MSI spatial proteomics including deparaffinization and epitope retrieval prior to PNGase F enzyme treatment for removal of proteoglycans. Tissues are matrix-coated and imaged for glycomics before being washed and prepared for peptide imaging following a similar protocol utilizing collagenase III that targets the skeletal ECM. Following matrix application, slides are imaged (D) with the timsTOF fleX mass spectrometer (Bruker).

Journal: bioRxiv

Article Title: Mass Spectrometry Imaging of the Subchondral Bone in Osteoarthritis Reveals Tissue Remodeling of Extracellular Matrix Proteins that Precede Cartilage Loss

doi: 10.1101/2024.08.03.606482

Figure Lengend Snippet: (A) Non-arthritic Cadaveric Joint (control) cadaveric tissue donors without diagnosed arthritis, osteoporosis, or fracture, and Osteoarthritis (OA) patients receiving knee replacement compose a cohort that is age, sex, and BMI matched and stratified by their clinical OARSI (arthritis) grade. Safranin-O/Fast Green histologic staining (B) of the tibial plateau of a non-arthritic donor (Control - top) and a representative patient with Osteoarthritis (OA - bottom) depict the loss of proteoglycan staining (red) in Medial OA, indicative of cartilage loss. Separation of tissue into medial and lateral sections allows for both site-matched comparisons with cadaveric tissue (medial OA vs Medial Control, blue arrow) and tissue-matched comparisons to healthier tissue from the same OA patients (OA Medial vs OA Lateral, red arrow). Formalin-fixed, paraffin-embedded (FFPE) tissue sections are prepared (C) for MALDI-MSI spatial proteomics including deparaffinization and epitope retrieval prior to PNGase F enzyme treatment for removal of proteoglycans. Tissues are matrix-coated and imaged for glycomics before being washed and prepared for peptide imaging following a similar protocol utilizing collagenase III that targets the skeletal ECM. Following matrix application, slides are imaged (D) with the timsTOF fleX mass spectrometer (Bruker).

Article Snippet: Deglycosylation was achieved by applying a 0.1 μg/μL PNGase F solution (N-Zyme Scientifics) via the TM Sprayer M3 (HTX Imaging, Chapel Hill, NC, USA), followed by incubation in custom humid chambers for 2 hours at 37°C.

Techniques: Control, Staining, Formalin-fixed Paraffin-Embedded, Imaging, Mass Spectrometry